This essential handbook on in vitro fertilization provides an overview of gamete biology combined with a practical account and full description of the advanced technologies and protocols used in the modern fertility laboratory. The first section of the book describes how gametes are produced, their interaction and the first stages of embryo development. This foundation in gamete biology is essential background for the clinical embryologist wishing to implement a successful IVF programme. The laboratory protocols described in the second half of the book are clearly illustrated and cover the full range of techniques applied in IVF: semen analysis and preparation, oocyte retrieval and embryo culture, embryo freezing and thawing, and ICSI. This is an indispensable and practical guide for clinical embryologists and indeed all clinical and laboratory staff involved in IVF.